Biotechnology Unit

Resistance to pests and diseases of plants mainly in the treatment of many other agronomic traits conventional breeding methods are often inadequate or for a long.

Developing very fast in the last 15-20 years through plant biotechnology techniques have the genes of agronomic importance, were isolated from different plant species or organism, can be readily transferred to cultivated varieties. While biotechnology is developing in the world, the working of biotechnology has started to considered important in our country after 1990s.In parallel, since 1996, Transtitional Zone Agricultural Research Institute biotechnology lab infrastructure of slowly began to be formed.

First in biotechnology laboratories and anther culture of wheat and barley has been initiated in the tissue. This rapidly evolving towards the 2000s was followed by Molecular Marker Techniques. Mainly on the use of molecular techniques for Plant Breeding selection continues. These techniques are PCR, RFLP, STMS, SSR.

1-PLANT BREEDING METHODS INTEGRATION TO MOLECULAR MARKER (PCR, RAPD, RFLP, SSR, STMS, STS)

A- Determinants of Anthracnose Resistance in Chickpea with Primera Screening of Varieties and Lines

B- Cereal Cyst Nematode Species with the PCR and RFLP Detection Method

2. DETERMINATION OF HIGH AND LOW MOLECULAR WEIGHT IN CEREALS

A-Page Electrophoresis of Wheat Varieties with Method and Determination of gliadin and gluten quality relations with electrophoregram

B-Protein Identification of the type identified by electrophoresis Page

3. TISSUE CULTURE TECHNIQUES AND ANTER of USE IN PLANT BREEDING

A- Anther culture

Wheat anther culture technique is used for  using double haploid plant production

TISSUE CULTURE TECHNIQUES AND ANTER of USE IN PLANT BREEDING

A- Anther-microspore culture technique isolation technique : Shorten the time for breeding a variety, develop 100 % homozygous lines , develop material for use in molecular studies, use of wild species.

YUKARI